SUMO - SAM-QC report: D:\Demo_R1.fastq.gz

Basics

Filename="D:\Demo_R1.fastq.gz_Aligned.out.sam.bam"
Total number of sequences: 7.600566 [MegaSeq]
Total number of bases: 0.943 [GigaBP]
Number of UNIQUE mapped sequences [Mega]: 3.304229 (43.47%)
Number of UNIQUE qualified mapped sequences [Mega]: 3.304229 ( 43.47%)
Number of bases [Giga]: 0.410 ( 43.47%)
AT-Content (%): 47.96
CG-Content (%): 52.03

Distribution of sequence length:

Score	-5	-10	-15	-20	-25	-30	-35	-40	-45	-50	-55	-60	-65	-70	-75	-80	-85	-90	-95	-100	-105	-110	-115	-120	-125
Count	0	0	0	0	0	14	64	107	58	51	62	37	18	14	6	3	3	3	3	981	1241	1089	13656	145342	7437814
%	0.000	0.000	0.000	0.000	0.000	0.000	0.001	0.001	0.001	0.001	0.001	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.013	0.016	0.014	0.180	1.912	97.859
Cumulative	0.000	0.000	0.000	0.000	0.000	0.000	0.001	0.002	0.003	0.004	0.005	0.005	0.005	0.006	0.006	0.006	0.006	0.006	0.006	0.019	0.035	0.049	0.229	2.141	100.000

Base composition:

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107	108	109	110	111	112	113	114	115	116	117	118	119	120	121	122	123	124	125
A (%)	28.61	27.43	25.42	24.46	25.77	24.86	26.29	25.72	25.02	25.75	25.12	25.69	25.79	25.65	26.06	25.47	25.54	25.79	25.23	25.43	25.98	25.98	25.53	26.13	26.40	26.58	26.22	25.27	26.61	26.24	26.67	26.68	26.06	25.89	26.82	26.43	26.70	25.80	25.82	26.54	26.98	26.99	27.10	26.17	25.98	25.72	26.61	26.65	27.03	27.05	27.21	26.06	25.95	26.18	26.56	26.45	26.94	27.22	26.20	25.99	26.30	26.26	26.89	27.01	26.66	26.12	25.89	26.43	25.97	25.99	25.63	27.22	26.21	26.85	25.78	26.20	26.49	26.08	26.31	26.29	26.54	26.21	26.18	27.29	26.52	27.05	25.87	25.72	26.98	26.33	26.47	26.61	25.70	26.96	25.93	26.98	26.12	26.91	25.92	26.36	26.51	26.58	26.55	25.86	26.50	26.52	26.14	25.77	25.82	26.95	26.00	26.73	26.86	26.21	26.81	26.43	27.47	26.83	27.12	26.53	26.52	26.59	27.61	26.86	13.78
C (%)	29.09	26.47	27.29	26.92	26.85	27.27	26.98	26.45	26.86	26.09	26.80	26.42	26.70	26.49	26.20	27.42	26.39	26.73	26.58	26.17	25.84	26.15	25.38	26.04	24.74	25.03	25.66	26.28	25.84	25.82	25.18	24.49	25.32	26.23	25.83	25.21	25.71	25.19	26.14	25.49	24.53	25.92	24.69	24.99	25.44	25.69	24.94	25.25	25.04	25.52	25.55	25.97	25.74	24.96	25.37	25.70	25.62	25.59	25.50	25.49	25.55	25.07	25.21	25.29	26.06	26.17	26.27	25.17	25.58	26.37	25.48	25.67	25.84	25.86	26.24	26.12	26.61	25.55	25.41	24.86	26.38	27.18	25.90	25.88	25.90	25.49	25.97	26.77	25.97	26.24	25.82	25.99	26.06	26.26	25.34	26.05	26.07	26.12	26.07	26.31	26.74	26.43	26.85	26.52	26.34	25.68	25.68	26.22	27.32	26.70	27.23	26.60	25.83	25.61	26.58	26.55	25.91	25.78	25.15	25.62	25.73	26.00	25.13	25.31	31.04
G (%)	28.29	24.45	25.04	26.01	25.16	26.34	25.59	25.98	26.63	26.17	26.93	26.09	26.07	26.28	26.30	25.65	26.58	26.33	26.38	26.79	26.70	26.10	27.34	26.07	27.25	27.34	26.67	27.14	25.79	25.71	26.35	26.59	27.28	25.81	25.71	26.11	25.39	26.62	26.16	26.18	26.69	25.83	26.27	26.56	26.39	26.13	26.21	25.20	25.73	26.26	25.79	25.67	26.36	26.65	25.91	26.07	26.05	25.96	26.67	26.72	25.59	26.26	25.44	26.12	26.00	26.34	25.15	26.59	26.57	26.00	26.59	25.35	25.56	25.60	25.80	25.36	25.43	26.25	25.63	26.15	25.58	24.55	26.53	25.32	25.90	25.69	25.88	25.52	25.24	25.79	24.91	25.30	26.69	24.88	26.76	24.89	25.67	25.53	26.23	25.59	24.75	25.09	24.49	26.19	26.23	26.04	26.58	25.89	25.30	25.48	26.00	25.31	25.52	26.47	25.65	26.19	25.80	26.23	26.13	26.33	25.92	26.94	26.89	24.64	30.46
T (%)	13.93	21.65	22.25	22.60	22.21	21.53	21.13	21.85	21.48	21.98	21.14	21.80	21.45	21.57	21.43	21.46	21.49	21.15	21.81	21.61	21.48	21.74	21.74	21.76	21.61	21.05	21.45	21.30	21.74	22.21	21.75	22.15	21.32	22.05	21.58	22.21	22.19	22.38	21.88	21.78	21.79	21.25	21.92	22.26	22.17	22.42	22.19	22.84	22.16	21.15	21.45	22.28	21.93	22.21	22.15	21.74	21.38	21.23	21.63	21.81	22.55	22.40	22.46	21.57	21.28	21.37	22.68	21.81	21.87	21.61	22.29	21.76	22.38	21.67	22.17	22.29	21.43	22.05	22.58	22.65	21.48	22.05	21.39	21.52	21.67	21.76	22.28	21.98	21.80	21.58	22.71	22.08	21.51	21.80	21.92	22.08	22.14	21.44	21.78	21.74	22.00	21.90	22.11	21.39	20.91	21.76	21.60	22.12	21.56	20.86	20.76	21.36	21.78	21.71	20.96	20.82	20.82	21.16	21.60	21.52	21.83	20.46	20.38	23.19	24.63
Other (%)	0.08	0.00	0.00	0.01	0.01	0.00	0.01	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.01	0.01	0.00	0.01	0.03	0.00	0.00	0.00	0.00	0.00	0.02	0.02	0.01	0.04	0.09	0.03	0.02	0.07	0.04	0.01	0.00	0.01	0.00	0.00	0.01	0.01	0.02	0.02	0.04	0.06	0.06	0.03	0.02	0.00	0.02	0.01	0.01	0.01	0.03	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.03	0.01	0.01	0.01	0.03	0.01	0.03	0.03	0.08	0.07	0.05	0.02	0.00	0.00	0.00	0.00	0.00	0.01	0.01	0.01	0.05	0.09	0.03	0.03	0.10	0.05	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.04	0.02	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.01	0.00	0.00	0.01	0.00	0.00	0.09

Distribution of Map-Quality values:

Score	0	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100
Count	932483	45366	0	98092	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4201541
%	28.221	1.373	0.000	2.969	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	127.156
Cumulative	28.221	29.594	29.594	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	32.563	159.719

Distribution of fragment length (PE):

Length (BP)	-0	-10	-20	-30	-40	-50	-60	-70	-80	-90	-100	-110	-120	-130	-140	-150	-160	-170	-180	-190	-200	-210	-220	-230	-240	-250	-260	-270	-280	-290	-300	-310	-320	-330	-340	-350	-360	-370	-380	-390	-400	-410	-420	-430	-440	-450	-460	-470	-480	-490	-500	-510	-520	-530	-540	-550	-560	-570	-580	-590	-600	-610	-620	-630	-640	-650	-660	-670	-680	-690	-700	-710	-720	-730	-740	-750	-760	-770	-780	-790	-800	-810	-820	-830	-840	-850	-860	-870	-880	-890	-900	-910	-920	-930	-940	-950	-960	-970	-980	-990	-1000
Count	0	0	0	0	0	1	15	164	2876	9005	19011	33533	47322	44568	46061	39867	31634	24005	17543	12507	8712	6003	4094	2882	1936	1295	894	622	450	333	224	203	176	184	155	184	179	143	136	143	120	112	104	80	105	120	93	114	99	145	140	122	147	114	126	107	125	107	86	67	64	71	51	51	59	52	47	55	55	53	57	67	95	153	102	60	38	38	44	36	35	40	26	41	36	31	24	33	25	44	45	37	56	51	76	79	63	56	49	46	7904
%	0.000	0.000	0.000	0.000	0.000	0.000	0.002	0.020	0.348	1.090	2.301	4.059	5.729	5.395	5.576	4.826	3.830	2.906	2.124	1.514	1.055	0.727	0.496	0.349	0.234	0.157	0.108	0.075	0.054	0.040	0.027	0.025	0.021	0.022	0.019	0.022	0.022	0.017	0.016	0.017	0.015	0.014	0.013	0.010	0.013	0.015	0.011	0.014	0.012	0.018	0.017	0.015	0.018	0.014	0.015	0.013	0.015	0.013	0.010	0.008	0.008	0.009	0.006	0.006	0.007	0.006	0.006	0.007	0.007	0.006	0.007	0.008	0.012	0.019	0.012	0.007	0.005	0.005	0.005	0.004	0.004	0.005	0.003	0.005	0.004	0.004	0.003	0.004	0.003	0.005	0.005	0.004	0.007	0.006	0.009	0.010	0.008	0.007	0.006	0.006	0.957
Cumulative	0.000	0.000	0.000	0.000	0.000	0.000	0.002	0.022	0.370	1.460	3.761	7.821	13.550	18.945	24.521	29.347	33.177	36.082	38.206	39.720	40.775	41.502	41.997	42.346	42.580	42.737	42.845	42.921	42.975	43.016	43.043	43.067	43.089	43.111	43.130	43.152	43.174	43.191	43.207	43.225	43.239	43.253	43.265	43.275	43.288	43.302	43.313	43.327	43.339	43.357	43.374	43.389	43.406	43.420	43.435	43.448	43.463	43.476	43.487	43.495	43.503	43.511	43.517	43.524	43.531	43.537	43.543	43.549	43.556	43.562	43.569	43.577	43.589	43.608	43.620	43.627	43.632	43.636	43.642	43.646	43.650	43.655	43.658	43.663	43.668	43.671	43.674	43.678	43.681	43.687	43.692	43.696	43.703	43.709	43.719	43.728	43.736	43.743	43.749	43.754	44.711

Distribution of LEFT clipped bases:

BP	0	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84
Count	6478720	205079	123590	55689	52905	42117	40026	36044	39808	36267	32212	31453	27917	26238	25554	25397	23477	21357	20764	19434	18070	17256	16547	14469	13537	13706	11452	11914	10112	10035	9690	7678	7731	6269	5938	5431	5157	4743	4312	3560	3217	2989	1991	2024	1919	1746	1679	1822	1441	1680	1290	1372	1257	1487	951	1488	877	855	918	992	665	690	726	616	516	344	461	328	240	219	189	170	179	248	193	210	173	171	126	111	123	115	74	26	3
%	85.114	2.141	1.268	0.966	0.917	0.588	0.579	0.494	0.593	0.507	0.452	0.429	0.399	0.392	0.381	0.362	0.324	0.290	0.289	0.280	0.296	0.272	0.236	0.199	0.208	0.190	0.175	0.167	0.146	0.131	0.133	0.112	0.097	0.070	0.077	0.072	0.055	0.052	0.048	0.049	0.038	0.033	0.022	0.022	0.021	0.019	0.021	0.019	0.024	0.022	0.021	0.018	0.017	0.015	0.014	0.015	0.012	0.012	0.009	0.010	0.008	0.008	0.007	0.006	0.005	0.004	0.003	0.003	0.002	0.001	0.002	0.002	0.002	0.002	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.000	0.000
Cumulative	85.114	87.256	88.524	89.490	90.408	90.996	91.574	92.068	92.661	93.168	93.620	94.048	94.447	94.839	95.221	95.582	95.907	96.197	96.486	96.766	97.062	97.335	97.571	97.770	97.979	98.169	98.344	98.511	98.657	98.789	98.922	99.034	99.131	99.201	99.278	99.350	99.405	99.458	99.506	99.555	99.592	99.626	99.647	99.670	99.691	99.710	99.730	99.749	99.773	99.796	99.816	99.834	99.852	99.867	99.881	99.895	99.908	99.920	99.929	99.939	99.946	99.954	99.961	99.967	99.972	99.976	99.979	99.982	99.984	99.985	99.987	99.988	99.990	99.991	99.993	99.994	99.995	99.996	99.997	99.997	99.998	99.999	100.000	100.000	100.000

Distribution of RIGHT clipped bases:

BP	0	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84
Count	6478720	205079	123590	55689	52905	42117	40026	36044	39808	36267	32212	31453	27917	26238	25554	25397	23477	21357	20764	19434	18070	17256	16547	14469	13537	13706	11452	11914	10112	10035	9690	7678	7731	6269	5938	5431	5157	4743	4312	3560	3217	2989	1991	2024	1919	1746	1679	1822	1441	1680	1290	1372	1257	1487	951	1488	877	855	918	992	665	690	726	616	516	344	461	328	240	219	189	170	179	248	193	210	173	171	126	111	123	115	74	26	3
%	85.240	2.698	1.626	0.733	0.696	0.554	0.527	0.474	0.524	0.477	0.424	0.414	0.367	0.345	0.336	0.334	0.309	0.281	0.273	0.256	0.238	0.227	0.218	0.190	0.178	0.180	0.151	0.157	0.133	0.132	0.127	0.101	0.102	0.082	0.078	0.071	0.068	0.062	0.057	0.047	0.042	0.039	0.026	0.027	0.025	0.023	0.022	0.024	0.019	0.022	0.017	0.018	0.017	0.020	0.013	0.020	0.012	0.011	0.012	0.013	0.009	0.009	0.010	0.008	0.007	0.005	0.006	0.004	0.003	0.003	0.002	0.002	0.002	0.003	0.003	0.003	0.002	0.002	0.002	0.001	0.002	0.002	0.001	0.000	0.000
Cumulative	85.240	87.938	89.564	90.297	90.993	91.547	92.074	92.548	93.072	93.549	93.973	94.387	94.754	95.099	95.435	95.769	96.078	96.359	96.632	96.888	97.126	97.353	97.571	97.761	97.939	98.119	98.270	98.427	98.560	98.692	98.819	98.920	99.022	99.105	99.183	99.254	99.322	99.384	99.441	99.488	99.530	99.570	99.596	99.623	99.648	99.671	99.693	99.717	99.736	99.758	99.775	99.793	99.809	99.829	99.842	99.861	99.873	99.884	99.896	99.909	99.918	99.927	99.936	99.944	99.951	99.956	99.962	99.966	99.969	99.972	99.975	99.977	99.979	99.983	99.985	99.988	99.990	99.992	99.994	99.996	99.997	99.999	100.000	100.000	100.000

Distribution of reads mapped to chromosome:

Chr	other (0)	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	X (23)	Y (24)	MT (25)
Reads	3326956	259722	79934	125569	31817	80370	66912	79669	148999	65754	71753	231356	159057	17070	39332	64330	92941	139003	14851	135431	49326	3241033	35142	73279	1613	2580475
%	29.67	2.32	0.71	1.12	0.28	0.72	0.60	0.71	1.33	0.59	0.64	2.06	1.42	0.15	0.35	0.57	0.83	1.24	0.13	1.21	0.44	28.91	0.31	0.65	0.01	23.02